mic tests against e coli atcc 25922 Search Results


clarithromycin against e coli atcc 25922 in vitro graphical abstract compound 16  (ATCC)
99
ATCC clarithromycin against e coli atcc 25922 in vitro graphical abstract compound 16
Figure 3. Membrane permeabilization assays of 16 in <t>E.</t> <t>coli</t> ATCC 25922. Polymyxin B was included as a comparator membrane-targeting agent. Both 16 and polymyxin B were used at concentrations ranging from 1/32X to 1/2X the minimum inhibitory concentration (MIC), with the MIC values of 16 and polymyxin B being 100 and 1.56 µg/ml, respectively. The compound vehicle was DMSO. (A,B) Impact of E. coli treatment with 16 (A) or polymyxin B (B) on the intensity of the fluorophore N- phenyl-1-naphthylamine (NPN) at a concentration of 10 µM. (C,D) Impact of treatment with 16 (C) or polymyxin B (D) on the percentage of E. coli cells stained with the fluorophore propidium iodide (PI) at a concentration of 50 µM.
Clarithromycin Against E Coli Atcc 25922 In Vitro Graphical Abstract Compound 16, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cefquinome against e coli  (ATCC)
99
ATCC cefquinome against e coli
Figure 3. Membrane permeabilization assays of 16 in <t>E.</t> <t>coli</t> ATCC 25922. Polymyxin B was included as a comparator membrane-targeting agent. Both 16 and polymyxin B were used at concentrations ranging from 1/32X to 1/2X the minimum inhibitory concentration (MIC), with the MIC values of 16 and polymyxin B being 100 and 1.56 µg/ml, respectively. The compound vehicle was DMSO. (A,B) Impact of E. coli treatment with 16 (A) or polymyxin B (B) on the intensity of the fluorophore N- phenyl-1-naphthylamine (NPN) at a concentration of 10 µM. (C,D) Impact of treatment with 16 (C) or polymyxin B (D) on the percentage of E. coli cells stained with the fluorophore propidium iodide (PI) at a concentration of 50 µM.
Cefquinome Against E Coli, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gram negative  (ATCC)
99
ATCC gram negative
Figure 3. Membrane permeabilization assays of 16 in <t>E.</t> <t>coli</t> ATCC 25922. Polymyxin B was included as a comparator membrane-targeting agent. Both 16 and polymyxin B were used at concentrations ranging from 1/32X to 1/2X the minimum inhibitory concentration (MIC), with the MIC values of 16 and polymyxin B being 100 and 1.56 µg/ml, respectively. The compound vehicle was DMSO. (A,B) Impact of E. coli treatment with 16 (A) or polymyxin B (B) on the intensity of the fluorophore N- phenyl-1-naphthylamine (NPN) at a concentration of 10 µM. (C,D) Impact of treatment with 16 (C) or polymyxin B (D) on the percentage of E. coli cells stained with the fluorophore propidium iodide (PI) at a concentration of 50 µM.
Gram Negative, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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test microorganisms  (ATCC)
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ATCC test microorganisms
Figure 3. Membrane permeabilization assays of 16 in <t>E.</t> <t>coli</t> ATCC 25922. Polymyxin B was included as a comparator membrane-targeting agent. Both 16 and polymyxin B were used at concentrations ranging from 1/32X to 1/2X the minimum inhibitory concentration (MIC), with the MIC values of 16 and polymyxin B being 100 and 1.56 µg/ml, respectively. The compound vehicle was DMSO. (A,B) Impact of E. coli treatment with 16 (A) or polymyxin B (B) on the intensity of the fluorophore N- phenyl-1-naphthylamine (NPN) at a concentration of 10 µM. (C,D) Impact of treatment with 16 (C) or polymyxin B (D) on the percentage of E. coli cells stained with the fluorophore propidium iodide (PI) at a concentration of 50 µM.
Test Microorganisms, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bacterial strains  (ATCC)
99
ATCC bacterial strains
Figure 3. Membrane permeabilization assays of 16 in <t>E.</t> <t>coli</t> ATCC 25922. Polymyxin B was included as a comparator membrane-targeting agent. Both 16 and polymyxin B were used at concentrations ranging from 1/32X to 1/2X the minimum inhibitory concentration (MIC), with the MIC values of 16 and polymyxin B being 100 and 1.56 µg/ml, respectively. The compound vehicle was DMSO. (A,B) Impact of E. coli treatment with 16 (A) or polymyxin B (B) on the intensity of the fluorophore N- phenyl-1-naphthylamine (NPN) at a concentration of 10 µM. (C,D) Impact of treatment with 16 (C) or polymyxin B (D) on the percentage of E. coli cells stained with the fluorophore propidium iodide (PI) at a concentration of 50 µM.
Bacterial Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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escherichia coli  (ATCC)
99
ATCC escherichia coli
Figure 3. Membrane permeabilization assays of 16 in <t>E.</t> <t>coli</t> ATCC 25922. Polymyxin B was included as a comparator membrane-targeting agent. Both 16 and polymyxin B were used at concentrations ranging from 1/32X to 1/2X the minimum inhibitory concentration (MIC), with the MIC values of 16 and polymyxin B being 100 and 1.56 µg/ml, respectively. The compound vehicle was DMSO. (A,B) Impact of E. coli treatment with 16 (A) or polymyxin B (B) on the intensity of the fluorophore N- phenyl-1-naphthylamine (NPN) at a concentration of 10 µM. (C,D) Impact of treatment with 16 (C) or polymyxin B (D) on the percentage of E. coli cells stained with the fluorophore propidium iodide (PI) at a concentration of 50 µM.
Escherichia Coli, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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e coli  (ATCC)
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ATCC e coli
Figure 3. Membrane permeabilization assays of 16 in <t>E.</t> <t>coli</t> ATCC 25922. Polymyxin B was included as a comparator membrane-targeting agent. Both 16 and polymyxin B were used at concentrations ranging from 1/32X to 1/2X the minimum inhibitory concentration (MIC), with the MIC values of 16 and polymyxin B being 100 and 1.56 µg/ml, respectively. The compound vehicle was DMSO. (A,B) Impact of E. coli treatment with 16 (A) or polymyxin B (B) on the intensity of the fluorophore N- phenyl-1-naphthylamine (NPN) at a concentration of 10 µM. (C,D) Impact of treatment with 16 (C) or polymyxin B (D) on the percentage of E. coli cells stained with the fluorophore propidium iodide (PI) at a concentration of 50 µM.
E Coli, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aerobic bacteria  (ATCC)
99
ATCC aerobic bacteria
Figure 3. Membrane permeabilization assays of 16 in <t>E.</t> <t>coli</t> ATCC 25922. Polymyxin B was included as a comparator membrane-targeting agent. Both 16 and polymyxin B were used at concentrations ranging from 1/32X to 1/2X the minimum inhibitory concentration (MIC), with the MIC values of 16 and polymyxin B being 100 and 1.56 µg/ml, respectively. The compound vehicle was DMSO. (A,B) Impact of E. coli treatment with 16 (A) or polymyxin B (B) on the intensity of the fluorophore N- phenyl-1-naphthylamine (NPN) at a concentration of 10 µM. (C,D) Impact of treatment with 16 (C) or polymyxin B (D) on the percentage of E. coli cells stained with the fluorophore propidium iodide (PI) at a concentration of 50 µM.
Aerobic Bacteria, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bacterial species  (ATCC)
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ATCC bacterial species
Figure 3. Membrane permeabilization assays of 16 in <t>E.</t> <t>coli</t> ATCC 25922. Polymyxin B was included as a comparator membrane-targeting agent. Both 16 and polymyxin B were used at concentrations ranging from 1/32X to 1/2X the minimum inhibitory concentration (MIC), with the MIC values of 16 and polymyxin B being 100 and 1.56 µg/ml, respectively. The compound vehicle was DMSO. (A,B) Impact of E. coli treatment with 16 (A) or polymyxin B (B) on the intensity of the fluorophore N- phenyl-1-naphthylamine (NPN) at a concentration of 10 µM. (C,D) Impact of treatment with 16 (C) or polymyxin B (D) on the percentage of E. coli cells stained with the fluorophore propidium iodide (PI) at a concentration of 50 µM.
Bacterial Species, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 3. Membrane permeabilization assays of 16 in E. coli ATCC 25922. Polymyxin B was included as a comparator membrane-targeting agent. Both 16 and polymyxin B were used at concentrations ranging from 1/32X to 1/2X the minimum inhibitory concentration (MIC), with the MIC values of 16 and polymyxin B being 100 and 1.56 µg/ml, respectively. The compound vehicle was DMSO. (A,B) Impact of E. coli treatment with 16 (A) or polymyxin B (B) on the intensity of the fluorophore N- phenyl-1-naphthylamine (NPN) at a concentration of 10 µM. (C,D) Impact of treatment with 16 (C) or polymyxin B (D) on the percentage of E. coli cells stained with the fluorophore propidium iodide (PI) at a concentration of 50 µM.

Journal: European Journal of Medicinal Chemistry

Article Title: Structure-activity relationships of potentiators of the antibiotic activity of clarithromycin against Escherichia coli

doi: 10.1016/j.ejmech.2019.05.075

Figure Lengend Snippet: Figure 3. Membrane permeabilization assays of 16 in E. coli ATCC 25922. Polymyxin B was included as a comparator membrane-targeting agent. Both 16 and polymyxin B were used at concentrations ranging from 1/32X to 1/2X the minimum inhibitory concentration (MIC), with the MIC values of 16 and polymyxin B being 100 and 1.56 µg/ml, respectively. The compound vehicle was DMSO. (A,B) Impact of E. coli treatment with 16 (A) or polymyxin B (B) on the intensity of the fluorophore N- phenyl-1-naphthylamine (NPN) at a concentration of 10 µM. (C,D) Impact of treatment with 16 (C) or polymyxin B (D) on the percentage of E. coli cells stained with the fluorophore propidium iodide (PI) at a concentration of 50 µM.

Article Snippet: M AN US CR IP T AC CE PT ED Graphical Abstract Compound 16 is associated with a 32-fold enhancement in the potency of clarithromycin against E. coli ATCC 25922 in vitro Graphical Abstract Compound 16 is associated with a 32-fold enhancement in the potency of clarithromycin against E. coli ATCC 25922 in vitro H2N H N NH2 16 H2N H N NH2 16 M AN US CR IP T AC CE PT ED -1- Structure-Activity Relationships of Potentiators of the Antibiotic Activity of Clarithromycin against Escherichia coli Gifty Blanksona, Ajit K. Parhia,c, Malvika Kaulb, Daniel S. Pilchb and Edmond J. LaVoiea aDepartment of Medicinal Chemistry, Rutgers, The State University of New Jersey, Piscataway, New Jersey, 08820, U.S.A. bDepartment of Pharmacology, Rutgers Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, U.S.A cTAXIS Pharmaceuticals, Inc., Monmouth Junction, New Jersey 08552, U.S.A. 1.

Techniques: Membrane, Concentration Assay, Staining

Figure 4. Assay for inhibition of the efflux of the fluorophore Hoechst 33342 (H33342) by 16 in E. coli ATCC 25922. When present, 16 was used at concentrations ranging from 1/32X to 1/2X MIC (3.13 to 50 µg/mL). All reactions were initiated by the addition of 50 mM glucose except where indicated. H33342 was used at a concentration of 10 µM. Compound vehicle was DMSO.

Journal: European Journal of Medicinal Chemistry

Article Title: Structure-activity relationships of potentiators of the antibiotic activity of clarithromycin against Escherichia coli

doi: 10.1016/j.ejmech.2019.05.075

Figure Lengend Snippet: Figure 4. Assay for inhibition of the efflux of the fluorophore Hoechst 33342 (H33342) by 16 in E. coli ATCC 25922. When present, 16 was used at concentrations ranging from 1/32X to 1/2X MIC (3.13 to 50 µg/mL). All reactions were initiated by the addition of 50 mM glucose except where indicated. H33342 was used at a concentration of 10 µM. Compound vehicle was DMSO.

Article Snippet: M AN US CR IP T AC CE PT ED Graphical Abstract Compound 16 is associated with a 32-fold enhancement in the potency of clarithromycin against E. coli ATCC 25922 in vitro Graphical Abstract Compound 16 is associated with a 32-fold enhancement in the potency of clarithromycin against E. coli ATCC 25922 in vitro H2N H N NH2 16 H2N H N NH2 16 M AN US CR IP T AC CE PT ED -1- Structure-Activity Relationships of Potentiators of the Antibiotic Activity of Clarithromycin against Escherichia coli Gifty Blanksona, Ajit K. Parhia,c, Malvika Kaulb, Daniel S. Pilchb and Edmond J. LaVoiea aDepartment of Medicinal Chemistry, Rutgers, The State University of New Jersey, Piscataway, New Jersey, 08820, U.S.A. bDepartment of Pharmacology, Rutgers Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, U.S.A cTAXIS Pharmaceuticals, Inc., Monmouth Junction, New Jersey 08552, U.S.A. 1.

Techniques: Inhibition, Concentration Assay